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Image Search Results
Journal: Human Cell
Article Title: Astragaloside IV alleviates senescence of vascular smooth muscle cells through activating Parkin-mediated mitophagy
doi: 10.1007/s13577-022-00758-6
Figure Lengend Snippet: AS-IV improved mitophagy and attenuated mitochondrial membrane potential loss in BLM-induced VSMCs. A After administration of AS-IV, MMP was identified by TMRM staining in BLM-induced VSMCs. Magnification, 200 × , scale bar = 50 μm. B TEM was used to observe the influence of AS-IV on the mitochondrial structure of BLM-induced VSMCs. C Flow Cytometer was used to assess the impact of AS-IV on the TMRM + fluorescent intensity in BLM-stimulated VSMCs. D P62 expression was examined by Western blot in BLM-induced VSMCs, which were given AS-IV, and the P62/TOMM20 value was determined. E expression of Parkin was measured by Western blot in BLM-induced VSMCs. LD low dose, MD medium dose, HD high dose. * P < 0.05, ** P < 0.05, *** P < 0.001
Article Snippet: The primary antibodies included p16 (Beyotime, AF1069, 1:1000), p21 (ProteinTech, 10,355–1-AP, 1:3000), DcR2 (Boster, A05136, 1:1500), P62 (ProteinTech, 18,420–1-AP, 1:3000),
Techniques: Membrane, Staining, Flow Cytometry, Expressing, Western Blot
Journal: Autophagy
Article Title: Deregulated MTOR (mechanistic target of rapamycin kinase) is responsible for autophagy defects exacerbating kidney stone development
doi: 10.1080/15548627.2019.1635382
Figure Lengend Snippet: Antibodies used in this study.
Article Snippet: Antibodies Product SQSTM1/p62 (sequestosome 1) MBL, PM045 phosphorylated SQSTM1/p62 (Ser 351; p-SQSTM1) MBL, PM074 LGALS3/galectin-3 (lectin, galactose binding, soluble 3) Santa Cruz Biotechnology, sc-23,938 mono- and polyubiquitinylated conjugated monoclonal antibody (FK2) BML, PW8810 LAMP1 (lysosomal-associated membrane protein 1) Abcam, ab24170 MAP1LC3 (microtubule-associated protein 1 light chain 3) MBL,
Techniques: Binding Assay